The objective of this study was to determine the effect of NBW3 on sterilization and cytotoxicity. Ozone nanobubbles showed significant bactericidal activity against oral infectious bacteria and were biocompatible with mammalian cells compared to conventional mouthwashes.

The objectives of this study were to evaluate the bactericidal activity of a novel antiseptic agent, ozone nanobubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The results suggest that NBW3 possesses potent bactericidal activity against representative periodontopathogenic bacteria and is not cytotoxic to human oral tissue cells.

Growing evidence of the connection between oral health and dementia risk. Studies show evidence of the connection between poor oral health and dementia risk. In 2020, scientists from the National Institute on Aging (NIA) found that those with symptoms of periodontal disease and oral infections were more likely to develop Alzheimer's disease or dementia.

Ozone is frequently used as a complementary therapy for various skin conditions, including infectious skin diseases, wound healing, eczema, dermatitis, psoriasis, axillary osmidrosis, diabetic foot, and pressure ulcers. Furthermore, several studies have reported the superior potential of ozone therapy for improving skin and gut microbiomes, as well as for anti-tumor and anti-aging treatment.

Ozonated water and other variants are forms of ozone used in dentistry, with a positive impact on patients with caries. Furthermore, the authors of the study described several effects of ozonated water, namely disinfectant, anti-inflammatory, activation of intracellular metabolism in the oral mucosa and dental wounds, improvement of local blood circulation, stimulation of regenerative functions, and hemostatic effect on capillary bleeding.

This study evaluated and compared the impact of ozonized water versus chlorhexidine mouthwash on the healing of intraoral inflammation associated with complete dentures. Ozonized water showed favorable results in reducing the area, intensity of inflammation, and degree of pain. Ozonized water has an antifungal effect and can be used as an alternative mouthwash for the treatment of denture stomatitis.

Ozonated water can be used as a pretreatment mouthwash for patients undergoing dental treatment to disinfect their oral cavity. Ozonated water used in the dental chair water supply, the pizo reservoir, and during the ultrasonic scaling procedure helps protect the dental surgeon and their auxiliary and technical staff from aerosol contaminants. It also prevents biofilm formation on the inlet and outlet drainage of the dental chair. Ozone sterilizes, leaving only oxygen and water as byproducts.

This study aimed to verify the bactericidal effects of ozonized water at different concentrations against staphylococcal species in vitro, as well as to evaluate the anti-inflammatory effects of ozonized water. These findings indicate that ozone administration may be a novel therapeutic approach for the management of allergic skin diseases.

Palmoplantar pustulosis (PPP) is a unique, chronic inflammatory skin disease. Its etiology and treatment are not clearly established. Recently, oral bacteria have been proposed as a possible causative factor for PPP. Ozone nanobubble (ONB) water, which exhibits potent sterilizing activity, has garnered interest as a new treatment method not only for periodontal disease but also for PPP.